FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9835315
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT741070.7534786633676704No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT574170.583784047587698No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT504980.5134355127415848No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT359360.365377214659622No Hit
CTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCTTTA331010.3365525150948394No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT285930.29071768418195043No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT256220.26051021243346045No Hit
CAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTT224350.22810657309908222No Hit
CCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTT222670.22639844275450252No Hit
GTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCC186240.18935844962769366No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA168580.1714027461245522No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC148780.15127120992057702No Hit
GCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCTT145310.14774310736361773No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC137900.14020903245091795No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC128920.13107866906143828No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT124240.12632030595868055No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG115960.1179016635461091No Hit
CTTTATTTCCCTTCTTTTTCTCTTTAGCTCGGCTTATTCCAGGGGTGTGT113180.11507511452353077No Hit
TGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCC107860.10966603509902835No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC100640.1023251415943465No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG98710.10036282518658528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12150.060.4666441
ACCGTTG14300.051.7069663
GACCGTT16400.044.7969362
CCGTTGA18750.039.4351774
GTTAGTT59300.029.6537531
CGTTGAC25600.029.623785
TAACCCC31650.029.2036279
ACCCGGC32250.028.5123145
TCTGTCA96350.027.892783
TCACCCG34400.027.5570053
TAGAGGC46300.027.2308443
CCGTATG26150.027.006924
ACTACCG26050.026.929197
CTACCGT28150.026.6035231
GTCAACC101200.025.7129756
CGCAAAA39400.024.6614231
CTGTCAA122900.024.6053334
CCCCACA214250.024.2686861
CCTAGAG54900.024.1738681
CTTCTGT119150.023.9078711
Produced by FastQC (version 0.11.5)