FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7634819
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAA11550.035.7779732
AGGCGAA26550.033.20661
GCGAAAA7300.031.8824523
CGTACGC13300.023.2134785
ACCCGTT16950.022.6983242
CGTACGA4550.021.92231454-55
CCGTACG14650.021.3985734
CCCGTAT13250.021.2203881
TGTCGAG9400.020.7173635
GTATCGA10800.020.6705764
ACGTACG4700.020.2120652-53
TTCGCGC7500.019.632657
CTCCGAT27850.019.5072841
CGCGCAT2502.1022788E-719.0623821
GGCGAAG22150.018.8706762
CACCCGT21600.018.7535021
CCGTTTA27750.017.4588534
GTACGCC18300.017.130556
CGCGTAA5300.017.084211
ATGCGCG37000.017.00954284-85
Produced by FastQC (version 0.11.5)