FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7634819
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG109550.1434873570676659No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA96420.12628983083947373No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT86140.11282520253590819No Hit
CAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGC79290.10385314962934943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT7800.050.4405481
GCATTCG21250.039.7061123
ACCGTTG12550.031.3495063
GACCGTT12650.031.101682
GCGGGTC38350.031.024492
CATTCGG27200.031.020814
CCGTTGA13850.029.4340934
ATTCGGG33750.024.579765
GTTAGTT45850.024.2954441
TCGGGCC35850.023.671187
CCGTATG13600.023.0041944
CGTTGAC18650.022.8758415
TACCCTA45500.022.7149879
CTACCGT14600.022.4022981
TCAGACG15400.022.1619552
CGTATGG14750.021.8539665
GTCTGTC57350.021.2434236
GGTCTGT57400.020.9766065
TTCGGGC40550.020.9260356
CGGGCCG42250.020.4225168
Produced by FastQC (version 0.11.5)