FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7702032
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGAA23400.031.1567611
GGCGAAA11850.028.0581252
GCGAAAA7300.024.0745283
TGTCGAG10100.023.5140575
ACCCGTT18150.022.5061262
CCGTACG13800.020.9956674
CGTACGA5900.020.93149654-55
CGTACGC14100.020.548955
GTCGAGG14900.020.0832126
ATGCGCG35900.019.64766584-85
TCGAGGG12900.019.5148437
CACCCGT23150.018.7312661
GCGCGTC38050.018.5374886-87
ACGTACG6150.018.53595752-53
TCGACCG4650.018.38647530-31
TACGCCA17800.017.344927
CTCCGAT27050.017.2637671
CTCGTTT31500.017.2453121
GTATCGA10250.017.1457614
GTACGCC17850.017.0302376
Produced by FastQC (version 0.11.5)