FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7702032
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG110860.1439360418133812No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA97650.12678472382353123No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT87390.11346356390105883No Hit
CAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGC78850.10237558088566757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT7700.044.939191
GCATTCG22500.040.4498943
GCGGGTC37650.030.0906582
ACCGTTG11400.029.9382483
CATTCGG31650.028.905814
GACCGTT12150.028.4803492
TCGGGCC34750.027.146337
GTTAGTT42150.026.990241
ACCCGGC25250.024.7807315
ATTCGGG37250.024.5602385
TCACCCG24550.024.521723
CTACCGT12850.023.9774681
TACCCTA45000.023.808159
CCGTTGA14200.023.3674474
CCGTATG12750.021.935294
CACCCGG28350.021.736614
CGGGCCG43100.021.4480138
CGACCTA6650.021.3846024
ACGTGCC24650.020.768522
GCTCGGA35150.020.634598
Produced by FastQC (version 0.11.5)