FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8095835
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC14100.023.2438345
CCGTACG14650.022.695424
AGGCGAA25900.022.2674621
GTATCGA9850.021.6997174
CCCGTAT13600.020.3270421
ACCCGTT16200.020.2307432
CACCCGT21700.019.7682341
TGTCGAG8950.019.6361565
GGCGAAA9800.018.9023722
TCCGTAC17650.018.5687273
GTACGCC19000.017.7493536
ATGCGCG37050.017.69166284-85
TACGCCA19100.017.6564247
CTCGTTT30150.017.3896351
CGTACGA5350.017.31245254-55
CTCGATT16250.017.012171
CGTATCG16900.016.8632893
TTCGAAA22250.016.6510776
CTCGAGT16350.016.6166021
CTCGTAT8900.016.6018621
Produced by FastQC (version 0.11.5)