FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8095835
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG116050.14334531274414558No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA102290.12634891891941968No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT91630.11318165451741544No Hit
CAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGC82900.10239833198181533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCG21800.040.8820763
CGACCGT7200.040.821421
GCGGGTC43350.030.7290272
CATTCGG30550.030.1038554
ATTCGGG35200.026.2619065
ACCGTTG11950.026.182293
GTTAGTT43550.024.274241
CGCGTTC6300.024.0806319
TCGGGCC38500.023.888347
ACCCGGC26350.022.8484845
GACCGTT13100.022.436342
TCACCCG27150.022.0004883
CGGGCCG43600.021.2030588
TACCCTA45400.021.1981819
TTCGGGC43750.020.9128656
CCGTTGA14400.020.7400444
GTCTGTC63650.020.4818216
CGCGGGT66950.020.4633241
GGTCTGT64650.020.4583195
TCAGACG15300.020.4495662
Produced by FastQC (version 0.11.5)