FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11171498
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA155760.13942624346349972No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG134990.12083428739816271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT23300.028.840941
GTATCGA19550.028.4260964
CGTATCG26450.024.7817273
CGTACGC13900.022.211445
CCGTACG14400.021.440214
CCGTATC31950.021.2590262
CTCCGAT43150.020.7645851
TGTCGAG12200.020.6345125
TATCGAA30250.020.5695175
ACGTACG6350.019.07412352-53
ACCCGTT14800.018.2932682
CTCGTTT37250.017.2724421
CGTACGA6950.017.08572254-55
TAAACCG92800.016.685839
TCCGTAC19150.016.122143
ATCGAAG49200.015.9293126
TACGCCA20300.015.9107637
CTCCGGT20450.015.847541
TCCGATT49050.015.7843522
ATGCGCG43350.015.77798684-85
Produced by FastQC (version 0.11.5)