FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11218912
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA155730.1388102518319067No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG134790.12014534029681309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA20200.028.9221974
CCCGTAT24650.027.4585061
CGTATCG26100.025.659973
CCGTATC30350.023.9447482
TATCGAA29850.022.2772335
ACCCGTT15300.022.0416682
CGTACGC15150.019.7517455
CTCGTTT36600.019.2746321
CTCCGAT45450.018.667731
CCGTACG16600.018.0264424
TGTCGAG14050.017.9174885
CTCGATT21050.017.888771
ACGTACG7100.017.39372352-53
CGTACGA7350.017.12521754-55
GTTCCGA19100.016.9103686
TAAACCG99000.016.6483759
CGAAAGG24850.016.6291648
CACCCGT21350.016.5211121
ATCGAAG48350.016.5040446
CCCGTCT32950.016.346641
Produced by FastQC (version 0.11.5)