FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11218912
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT237260.21148218294251708No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG187910.16749396019863602No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT164840.14693046883690683No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC157920.14076231278041934No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT140530.12526170095638506No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA126150.11244405874651658No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC125470.11183793936524325No Hit
CTCATCCACAATGATTTCTCCAGTGCTCATCTTGTTCTCGAGTTTTCTCT113510.10117736907108282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12950.051.2424281
GCGGGTC53900.034.208512
ACCGTTG19250.033.4874083
CATTCGG22700.031.947654
GCATTCG23550.030.7945523
CCGTTGA21200.030.630784
GACCGTT21500.030.4238382
CCGTATG28350.027.5869854
CTACCGT28550.027.5596311
CGTATGG30650.025.8262465
GCCTATC51350.025.1998236
ACCCGGC36700.025.0559565
ACGACGT9300.024.974027
TCACCCG35200.024.9116483
GTTAGTT61800.024.6966931
ACTACCG26800.024.5841567
CGTTGAC27400.023.8728245
CGCGGGT82550.023.024951
ACCGTAT34650.022.7079663
GGATTCG29400.022.571278
Produced by FastQC (version 0.11.5)