FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004827882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3997486
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG321050.8031297670585963No Hit
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA104760.2620647076687698No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT104500.26141429888685036No Hit
CCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA100460.2513079470447176No Hit
CTCCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGA81300.20337782296173143No Hit
CTCAGGTTCTTGTTTTGCACAGCAGTCAGCCATTTCACCATAGGTTTCAC77620.19417203712533326No Hit
GGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAA77280.1933215025643617No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA76720.19192062211099678No Hit
CTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATCT58050.14521626842470492No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG53400.13358395751729962No Hit
CTCCAAAAAGGGTATGAAGTGATTTGTCACAATTTTCAGCTGACTCATCA53360.13348389462777355No Hit
CCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGT53350.13345887890539204No Hit
CTTTGAAATTTTCTTCTCCCAAATCTTTAAACCGATGAGCAACCTCACTC52660.1317327940610674No Hit
CACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAAATT52230.13065711799866217No Hit
CTTCAAATGGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAG51800.12958144193625692No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT48760.12197666233227583No Hit
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT48130.12040067182224029No Hit
CTTCAGGATGTTTACAACATTTGCTGCCCACTTTTCCTAGGTTTCTTGAG47530.11889972847934926No Hit
CTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAAGTTCATCGAGCTT47430.1186495712555341No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT47250.1181992882526668No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA44790.11204542054681367No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT41620.10411543655187284No Hit
CGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTT40160.1004631410841714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)