FastQCFastQC Report
Fri 10 May 2024
EGAF00004827886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4062764
Total Bases410.3 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG329750.8116395636074356No Hit
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA108140.2661734720500625No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT105930.2607338255433985No Hit
CCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA103150.25389119328614707No Hit
CTCCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGA82010.20185765158891827No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA80220.19745178405637148No Hit
CTCAGGTTCTTGTTTTGCACAGCAGTCAGCCATTTCACCATAGGTTTCAC79460.19558113638892144No Hit
GGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAA79300.195187315827353No Hit
CTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATCT60150.14805191736463158No Hit
CTTTGAAATTTTCTTCTCCCAAATCTTTAAACCGATGAGCAACCTCACTC55170.1357942523858142No Hit
CTCCAAAAAGGGTATGAAGTGATTTGTCACAATTTTCAGCTGACTCATCA54910.13515429397326548No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG54730.13471124584150101No Hit
CCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGT53030.13052690237483644No Hit
CACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAAATT52860.13010846802816997No Hit
CTTCAAATGGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAG52190.12845934442660217No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT51080.1257272142807212No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT48550.11949992665092041No Hit
CTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAAGTTCATCGAGCTT48530.11945069908072435No Hit
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT48450.11925378879994014No Hit
CTTCAGGATGTTTACAACATTTGCTGCCCACTTTTCCTAGGTTTCTTGAG48060.11829385118111709No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA46580.11465101098660911No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT42170.10379633175837927No Hit
CGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTT41680.10259025628857595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)