FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12958437
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1589971.2269766793634138No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA366310.2826806967537829No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG354110.2732659810747238No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC269720.2081423863078549No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT202070.1559370161694655No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA175590.1355024529578683No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA174670.13479249079190647No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT168890.1303320763144506No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT167550.12929800098576702No Hit
GACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGG155370.11989871926683751No Hit
GGCAAGACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGC152810.11792317237024805No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC148170.11434249362017965No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT141490.10918755093689154No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC141110.10889430569442904No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC137690.10625509851226657No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG129740.10012009936074852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC240600.066.548895
TCACCCG244850.065.435613
CACCCGG253900.063.1034664
CGGCCCG287750.055.7463728
CTCACCC301850.053.094782
CCAGTAG69800.049.826715
CCCGGCC332850.048.3487976
CCGGCCC358100.045.4178927
CCTCACC364800.044.505481
GTAGCAT81650.043.1084988
TAGCATA81700.042.907369
CGGACAC243700.037.86041612-13
GACACGG251550.036.79115714-15
CAGTAGC95250.036.7031756
CCCGGAC255100.036.22484610-11
CGGACAG258200.035.79697818-19
CACGGAC264000.034.95689416-17
GGCCCGG476150.033.738629
CCGGACA249100.033.49302312-13
ACGGACA253100.032.95278518-19
Produced by FastQC (version 0.11.5)