FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004827891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12955470
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1587541.2253820201042493No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA362650.2799203734021228No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG350410.27047262662026156No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC270660.20891561633811817No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT204390.15776347751181546No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA176260.13605064115775034No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA174250.1344991729362192No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT168130.1297752995452886No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT167630.1293893621767485No Hit
GACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGG158290.12218005213241974No Hit
GGCAAGACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGC154170.11899992821564945No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC148850.11489355461438296No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT143040.11040896239194718No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC142910.11030861867612676No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC138640.10701271354879446No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG132470.10225024642100981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)