FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004827892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12952389
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1050210.8108233932751711No Hit
CCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGT371480.28680423356648727No Hit
GGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC314140.24253440813119498No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG306970.23699874980592384No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC279230.21558185134804092No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG278460.2149873664232907No Hit
GGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTT276060.21313442639809538No Hit
GTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT261190.2016539188253225No Hit
CGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTA261130.20160759532469263No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT213790.1650583533277143No Hit
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT208030.16061129726724546No Hit
GGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCAC206760.15963078317057958No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT198500.15325358125053223No Hit
CGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTT195430.15088336213496983No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC190080.14675284999547186No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC185330.14308557286227275No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA175970.13585910676401086No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT174280.1345543281629358No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG168560.1301381544362202No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG160690.12406205527026715No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT150530.11621794249694015No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC148550.11468926697615398No Hit
GGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA146500.1131065473712996No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA145020.11196390102242915No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT139970.10806500638608058No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG138120.10663669844999252No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC138030.10656721319904769No Hit
GGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATA138010.10655177203217105No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGA137840.10642052211371972No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC134560.10388817074595273No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC134250.103648832659365No Hit
GCTCAGGCGAGCTACTGCCCATGCTTTGAAAGCTCTTTCTCCAAATTTTT129750.1001745701121237No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC129550.10002015844335743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)