FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12952389
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1565521.208672778434928No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA358390.27669798984573424No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG350350.27049064076132984No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC267520.2065410481417752No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT199970.1543885070159644No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA174460.13469329866482546No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA171420.13234624129957803No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT166400.1284705084135444No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT165770.1279841116569306No Hit
GACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGG152700.11789330910305428No Hit
GGCAAGACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGC149720.11559257523843672No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC146220.11289037103502683No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT143220.11057419600353263No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC142180.10977125532594798No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC135760.10481464075855042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC247600.065.64135
TCACCCG253900.064.218253
CACCCGG260200.062.5356524
CGGCCCG287850.056.4324658
CCAGTAG68700.053.286665
CTCACCC311200.052.451792
CCCGGCC336750.048.323296
CCGGCCC362950.045.3240787
TAGCATA81150.045.1661539
GTAGCAT82150.045.0251438
CCTCACC371050.044.629771
CAGTAGC93150.039.2961466
CGGACAC246750.038.7010312-13
CCCGGAC253450.037.50702310-11
GACACGG256150.037.19819614-15
CGGACAG260050.036.4364118-19
CACGGAC270750.035.0771816-17
GGCCCGG470800.034.4929669
CCGGACA250850.033.4835312-13
GCCAGTA110800.033.2110674
Produced by FastQC (version 0.11.5)