FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793069
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG41660.14915492599717373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG5050.032.916333
CGTCGGC1901.2732926E-1129.9959343
CTCCGGT9400.028.8613071
GTATCGA4650.027.5769064
CCGTATC7150.026.5712532
CCCGATT2155.638867E-1126.5651231
CCCGTAT6500.025.6285291
CGAGTGA4100.025.4843523
TCGAGTG4200.024.8789182
CCCGTCT5400.024.6793271
CCGGGGC4550.024.0594371
CGCACTA1652.3897264E-623.076771
CCCGTAC2303.5488483E-922.763231
TTAACGC2303.6525307E-922.7065877
CCCGGGT3450.022.0734331
CCGGGTT6800.021.6981031
CGGTTTG7900.021.6426354
CTCGAGT6650.021.4718071
TCGACGA12400.021.4510144-45
TAAGCCG12550.021.00801866-67
Produced by FastQC (version 0.11.5)