FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2844588
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC48310.1698312725779621No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC41940.147437871494923No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA34760.12219695787228238No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC34710.12202118549329465No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATACCGCTCACACCT32780.11523637166436757No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT30720.10799454965007235No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT30120.10588528110221937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT3150.063.3328551
ACCGTTG4900.042.6347353
CCGTTGA4950.042.2040824
CGGCCCG3350.039.6990938
GACCGTT5100.039.0994642
CTACCGT10850.034.1472051
CCGTATG10900.033.5406344
CGTATGG12100.030.6082945
CACGCCG2550.029.7911174
ACCCGGC4850.029.3703735
ACCGTAT12350.029.2182123
TACACGC2650.028.6659152
GCTCGGA7650.028.5603668
CGCAAAA10450.028.1816041
AGGCTCG8100.026.9660916
ACGCCGC3000.026.9065235
GGCTCGG8750.024.965537
TAACCCC11500.024.787229
ACTACCG7100.024.0788157
AACCCAC25400.023.9279885
Produced by FastQC (version 0.11.5)