FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13129603
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA356300.27137149539098787No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG221570.1687560545433095No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC217370.1655571764051053No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT199840.15220566836636262No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG199240.15174868577519063No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC180410.13740704878890855No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT165140.12577684184358048No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT149130.11358302303580695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTC42100.035.0950432
GTTGACT103800.034.555361
CGACCGT19150.032.746961
ACTACCG69200.029.3071377
CTACCGT70150.028.842548
TTGACTA129550.026.4769042
AGTAGGT57600.026.145841
GACTATT135850.025.3537674
GGTCTAC61700.024.3305995
GACCGTT25650.024.2633842
TGACTAT147300.023.3830453
ACCCGGC38550.023.2910085
TAGGTCT65800.023.0319423
ACCGTTG28100.022.655013
AGGTCTA69000.022.5145264
ATCACAT99250.021.7315711
TCACATG102400.021.666282
CTATAAC58550.021.4154386
TACCGTA93800.021.2162869
CATAAAT89900.020.873881
Produced by FastQC (version 0.11.5)