FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13151508
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA351710.2674294080952542No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG221360.16831529889956345No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC213840.1625973234400192No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT197360.15006644104995412No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG197180.14992957461608206No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC177180.13472219307474093No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT160760.12223693282929987No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT145000.11025351617472308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACT105050.036.517921
GTAGGTC44450.033.8602182
ACTACCG67400.031.389197
CTACCGT69350.030.1605878
CGACCGT16850.028.7402781
GACTATT133100.027.932354
AGTAGGT55250.027.9281251
TTGACTA132900.027.7943742
TGACTAT144600.026.0061283
TAGGTCT65050.023.503223
TACCGTA90450.023.1777349
ATCACAT99850.022.8235861
GGTCTAC66350.022.5419645
ACCGTTG23750.022.3909843
GACTAAG41600.022.2568325
ACCCGGC41100.021.9499665
TCACATG103900.021.6146322
AGGTCTA73000.021.529254
GACCGTT24000.020.9699252
CGCAAAA40200.020.6682031
Produced by FastQC (version 0.11.5)