FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9868376
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG327260.331624980645245No Hit
CCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA103510.10489061219394154No Hit
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA101560.10291460317280168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG27300.033.0513043
CCCGTCG14750.032.6026571
GTATCGA21250.031.7341634
CCCGTAT24650.031.4843251
CCGTATC31450.029.143152
CTCCGGT40500.027.274491
CCGGGGC26250.025.212111
CGTACGC1151.284642E-424.7771345
TCCGGTT39500.024.6465782
CCGGTTT37300.024.0630463
CCCAAAT180650.024.0370181
CGGTTTG37200.023.8724124
CGTCGGC19450.023.6837123
CGCGGTA1252.2216207E-422.8541411
CCCGGTT24050.022.7670351
TATCGAA31750.022.4359875
CGCGGCG2154.440517E-822.0881424
TCGACGA99700.022.07817844-45
CTCCGTT23700.021.696971
CCCGTCT18000.021.6902731
Produced by FastQC (version 0.11.5)