FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9868376
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT256680.26010358745957796No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT226220.22923731321141388No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC185610.18808565867372706No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC184630.18709258747335933No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT175160.17749627699633658No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT141990.14388385687776792No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT141800.14369132266545173No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA140800.14267798470589285No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATACCGCTCACACCT131290.13304114071048773No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG129060.13078139706067138No Hit
GGGCAGGAAAGCTCCATGCACATAGCCCAGCAAAGAGCAACACAGAGCTG128270.12998086007261986No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT127260.12895738873346538No Hit
CCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTT123820.12547150615258276No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT109390.11084903939614786No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC106110.10752529088879467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCCG28750.056.190288
CGACCGT11700.054.8214341
ACCCGGC32700.050.85275
CCGGCCC35500.046.5717547
CACCCGG36700.045.438714
CCCGGCC37350.044.51696
CGCAAAA34450.042.4779971
TCACCCG42350.039.6010063
ACCGTTG17050.039.0111353
GACCGTT17750.036.4020162
GGCCCGG46650.036.263459
CCGTATG35450.030.0204164
TAACCCC47900.029.1663869
CACGCCG5050.029.1645474
CCGTTGA22700.029.092024
CGGACAC34100.028.778312-13
CTACCGT37600.028.4313321
CGTATGG37700.027.977275
GTAGGTC32150.027.1907862
GCTCGGA25950.027.0984548
Produced by FastQC (version 0.11.5)