FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10002223
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG336660.3365851771151273No Hit
CCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA105320.10529659256747226No Hit
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA104880.10485669035773348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA20800.036.723864
CGTATCG26400.036.6616553
CCCGTAT24850.035.7771341
CCGTATC30750.033.3283652
CTCCGGT41350.026.4449441
TCCGGTT39250.025.9898722
CCGGGGC27150.025.5666241
CCCGTCG13500.024.652141
TATCGAA32900.024.2269765
CCCAAAT176750.023.832231
CGCGTGA1000.001645759623.7717061
CCGGTTT39200.023.4801853
ATCGAAG42500.022.3158866
CGGTTTG39400.022.156824
TCGACGA100450.021.63659144-45
CGTACGC1100.002904304521.5582397
CTCCCGT20750.021.3086151
CGTACGA2700.021.10010754-55
GACGAAA103650.020.8672946-47
TAAGCCG105200.020.73112966-67
Produced by FastQC (version 0.11.5)