FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13047954
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA391610.3001313462631766No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG185740.1423518200631302No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT174380.13364547422530768No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA155320.11903782002910188No Hit
GTTGAGGTTGCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTG151810.11634774310209861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG35150.037.9694678
GCCCTCT148250.031.225571
CGTGCCG78550.030.11797368-69
CTATCGG82750.028.64623858-59
CCCTCTT157100.028.600162
GAACTCT148100.028.2215679
ATCGCGT6450.027.98202194-95
ATCGGTC86050.027.63195660-61
GTCCGTT12350.027.4079931
TGAACTC154700.027.1710518
CCGGTAT87400.026.9023772-73
CCTTACG88800.026.87818340-41
ACGGTAC89200.026.78371444-45
ACTATCG79550.026.33500958-59
CCTCTTG167350.026.2240493
CTCTTGA162500.026.1883494
GACTATC90750.026.17259856-57
TATCGGT81650.025.74633860-61
CGCAATC28900.025.4734272
TTACGGT97650.024.49085842-43
Produced by FastQC (version 0.11.5)