FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13036326
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA390320.29940951154489387No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG184630.1416273265949317No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT174280.1336879731298527No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA154830.11876812531383459No Hit
GTTGAGGTTGCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTG150730.11562306741945544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG37700.041.321828
GTCCGTT12100.036.239561
GCCCTCT152400.030.399171
CGTGCCG81500.028.88182468-69
CTATCGG83600.028.18430358-59
CCCTCTT159650.028.083422
TGAACTC152450.027.2912628
GAACTCT152950.027.1709929
CTCTTGA158500.026.9087814
ATCGGTC88550.026.66278860-61
ACGGTAC89150.026.63798944-45
CCGGTAT87400.026.54957272-73
CCTCTTG167400.026.4427953
CCTTACG90750.026.27306240-41
ACTATCG80700.025.57691458-59
GACTATC93100.025.536456-57
ATCGCGT5650.025.2220694-95
TATCGGT84850.024.3542760-61
TTACGGT98000.024.35362242-43
TCGATTG43300.024.35073324-25
Produced by FastQC (version 0.11.5)