FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13514697
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG494580.3659571502046994No Hit
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA222520.16465038024899856No Hit
CCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA173020.12802358795021448No Hit
CCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGT165290.12230388887002056No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG163100.12068343078649858No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT147040.10880007150733753No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT138620.10256981714055448No Hit
CGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTA136740.10117873896839863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC3100.052.0874525
CCGTACG5500.030.2218634
CGCGGCG8450.029.2255384
CGCGTAT1652.588422E-928.8538361
CTCCGGT52050.028.446391
CCCGTAT32400.027.3309921
GTATCGA26300.027.2669834
TCCGGTT48500.026.7325272
CCGGGGC33150.025.42011
CGTATCG40850.025.2280433
CCCAAAT246500.025.1467321
GTACGCC6700.024.8045866
CGGTTTG47100.024.4011524
TCCGCGG10500.023.9720752
CCGTATC47150.023.670382
CCGGTTT50000.023.2708343
CCGGGTT35450.022.830751
TCGACGA151050.022.29168944-45
CCCGTCG10650.021.904531
GACGAAA155200.021.67951446-47
Produced by FastQC (version 0.11.5)