FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13514697
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT351610.26016861495303967No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT327400.24225478381054347No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT307490.22752267401925474No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG263130.1946991486379606No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC246670.18251981527961744No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC243380.18008542847834472No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA239000.1768445123113008No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT214350.1586051096817043No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT208020.15392131987864766No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATACCGCTCACACCT190490.1409502558584924No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA181430.134246442965018No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT172330.12751303266362538No Hit
CTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCTTTA165660.122577664893264No Hit
CCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTT152740.11301770213568237No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC143270.10601051581104631No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA139100.10292498603557297No Hit
CAGGAAAGCTCCATGCACATAGCCCAGCAAAGAGCAACACAGAGCTGAAA137480.1017262910148855No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC135870.10053499534617757No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC135840.1005127972902389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT21300.066.022681
CGGCCCG27300.048.382218
GACCGTT30000.047.03362
ACCGTTG30450.046.338523
CCGTTGA31650.044.7317164
CGCAAAA55550.040.881321
ACCCGGC33750.038.9917375
CCGGCCC39000.033.9863557
CACCCGG39250.033.5284234
TAACCCC68150.033.0496989
CCGTATG41550.032.4728434
CTACCGT42900.032.1160241
CGTATGG42800.031.7459895
TCACCCG43600.031.3818863
CCCGGCC45100.029.599336
ACCGTAT47250.029.3598653
ACATGCC98000.028.359782
CGTTGAC56650.026.919795
GCCTATC104950.026.7973216
GCTCGGA37450.026.5153888
Produced by FastQC (version 0.11.5)