FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13641937
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG513140.3761489295838267No Hit
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA228330.16737359218122763No Hit
CCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA177020.12976163135777566No Hit
CCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGT169400.12417591431480735No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG166060.12172758164767951No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT149410.10952256999867394No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT140870.10326246192164645No Hit
CGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTA138560.10156915399917182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC2950.048.24525
CTCCGGT53950.031.4585531
CCCGTAT30300.028.2417661
TCCGGTT51450.028.1251492
GTATCGA25250.027.4312974
CCCGTCG11400.026.6892951
CCGTACG5350.026.6024974
CGCGGCG8850.026.2668324
CCGGGGC36500.025.9192371
TCCGCGG9750.025.7900182
CCCAAAT246350.025.5310861
CGGTTTG50300.025.2767774
GTACGCC6200.025.2392566
CGTATCG38200.024.3415133
CCGGTTT54400.024.0693873
CTCCGCG13350.023.5030731
CCGTATC43350.023.0926462
TCGACGA150400.022.53456744-45
CTCCGAT88400.022.4256881
GACGAAA153850.022.04425646-47
Produced by FastQC (version 0.11.5)