FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11998627
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA343980.2866828012905143No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC215940.1799705916351929No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG201910.16827758709392335No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG198260.165235572370072No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT155210.12935646720245575No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC152400.12701453257943596No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT148250.12355580350985158No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT146820.12236400048105504No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA140410.11702172256875724No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA127350.10613714385820977No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG124400.10367852921838473No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATACC120040.10004478012359248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTC41650.038.326992
CGACCGT18800.036.389951
GTTGACT100050.034.5218541
AGTAGGT51950.030.9106391
TTGACTA117650.027.782862
ACCGTTG25800.027.0693653
GGTCTAC60600.026.8118445
GACCGTT25750.026.7529222
CGCAAAA37500.026.6050991
TAGGTCT62250.026.2542463
GACTATT125600.026.0240944
AGGTCTA65750.025.5067884
ACCCGGC37500.025.4646665
CGGCCCG39150.024.871148
TGACTAT132400.024.7235983
ACTACCG46850.024.3318587
CTACCGT46900.024.1033658
ATCACAT90100.023.6754551
TACTATA57850.023.4055214
CTATAAC58250.022.4233916
Produced by FastQC (version 0.11.5)