FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13967161
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA458160.32802657605221275No Hit
GGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTT287780.20604044014384884No Hit
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT283650.20308350422823937No Hit
CGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTT251190.1798432766687518No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG205810.14735277985268447No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT178570.12784989018169118No Hit
CACTGCTTCTTATCATTTAAGGTGTATACTCCATCTCCTTCTGTGCGCAG156110.11176931374958733No Hit
GGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATA146660.1050034434342097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG26800.038.4573558
CCGGGGC98000.030.400011
GAACTCT176200.029.00429
TGAACTC182450.028.3750828
GCCCTCT199650.028.172961
CCCTCTT208100.027.5479742
CTCTTGA189650.027.3729634
CCTTACG112150.026.66043940-41
CTATCGG112150.026.57992258-59
CGTGCCG111950.026.45871268-69
ACGGTAC115350.025.94114344-45
CCTCTTG209850.025.575493
GACTATC117600.025.50864256-57
TTGAACT207300.025.4776847
GTCCGTT12400.024.9868451
CTTGAAC211350.024.8995766
ACTATCG107250.024.6494458-59
ATCGGTC122050.024.63926160-61
CCGGTAT120150.024.4718372-73
TCTTGAA219100.024.2789615
Produced by FastQC (version 0.11.5)