FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13967161
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGG305720.21888485426637527No Hit
GGCAAGACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGC290590.20805230211064368No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA273470.19579497938056273No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT265560.1901316953388022No Hit
CGTGCCTCCTGGTCTCAGTATGGCGCTGTCCTGGGTTCTTACAGTCCTGA202600.14505453184079428No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT199950.1431572242920376No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC194540.13928385303212298No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG180590.1292961397094227No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG178270.12763510064786968No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT174170.12469964368564235No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG172710.12365433462104432No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT161720.11578587803204961No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT145120.10390085716059261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT17450.038.6638571
ACTACCG62100.031.1309327
CTACCGT63150.030.012018
GTAGGTC35400.029.5278382
GTTGACT109450.028.2603451
GACCGTT24700.028.0845932
ACCGTTG25400.027.497673
TTGACTA102750.027.3748722
TATAGCC36450.026.8447287
GACTATT109400.025.9715394
TACTATA40450.025.2542064
AGTAGGT43100.024.2525651
CATAAAT77050.023.371081
GCAAGAC134850.023.3601072
ATCACAT81600.022.8248621
TCACATG83300.022.7583182
CCGTTGA30750.022.5590884
CGTGCCT94000.022.1390251
ACTATAG50250.021.9353965
TGACTAT132950.021.7998623
Produced by FastQC (version 0.11.5)