FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13943851
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA457650.32820918697424406No Hit
GGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTT288750.20708052603258595No Hit
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT282230.2024046298257203No Hit
CGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTT253140.18154238739355433No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG208280.14937050030153076No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT175500.1258619301081172No Hit
CACTGCTTCTTATCATTTAAGGTGTATACTCCATCTCCTTCTGTGCGCAG153540.11011305269971688No Hit
GGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATA146110.1047845390774758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG26450.038.068758
CCGGGGC96000.029.093011
GTCCGTT12150.027.4589561
GAACTCT168600.026.9032159
TGAACTC172300.026.4081928
CGTGCCG103700.025.99677768-69
CTCTTGA180500.025.7347564
CTATCGG105750.025.42575858-59
CCTTACG106850.025.35993240-41
ACGGTAC108850.024.91610344-45
GCCCTCT200100.024.818921
GTCCGGT29100.024.403691
GACTATC111250.024.3603456-57
CCCTCTT203900.024.2954792
TCGATTG27100.024.0109724-25
CCTCTTG200550.023.9908263
CCGGTAT112050.023.9514872-73
ATCGGTC114650.023.5764960-61
TTGAACT198150.023.466437
GGCGGTC48700.022.9985871
Produced by FastQC (version 0.11.5)