FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13943851
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGG308120.22097195387414853No Hit
GGCAAGACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGC294580.21126158046295818No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA273330.19602188807094967No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT259210.18589556070270688No Hit
CGTGCCTCCTGGTCTCAGTATGGCGCTGTCCTGGGTTCTTACAGTCCTGA205480.14736244671576024No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT199760.143260280104829No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC194940.139803559289324No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG178770.12820704983149922No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG178330.12789149855373527No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT175330.12574001256898112No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG170400.12220440393403516No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT161020.11547742442170389No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT144870.10389525820377742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT17550.036.2722851
GTAGGTC34450.032.26862
ACTACCG58800.030.3711437
CTACCGT61950.028.827168
GTTGACT109350.027.6737441
TATAGCC33450.027.5458537
AGTAGGT40650.027.4634191
ACCGTTG23800.026.5479683
TTGACTA104000.026.4940382
GACTATT107250.026.0454544
GACCGTT25600.024.4955642
ATCACAT84500.024.1183621
TCACATG83850.023.5691412
CGTGCCT92850.023.0750081
CATAAAT73800.022.7229981
GCAAGAC132950.022.2614362
TACTATA41650.022.1278214
CGCAAAA41500.021.63521
GCTCGGA52200.021.4732048
TAGGTCT52700.021.3645783
Produced by FastQC (version 0.11.5)