FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13951969
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA461630.33087086130996995No Hit
GGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTT288230.2065873282831979No Hit
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT282740.20265239981539523No Hit
CGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTT246270.1765127201759121No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG208930.14974947263715968No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT178040.1276092284895415No Hit
CACTGCTTCTTATCATTTAAGGTGTATACTCCATCTCCTTCTGTGCGCAG154960.11106676054111071No Hit
GGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATA146890.10528263071685438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG26350.034.787168
CCGGGGC99500.029.409691
GAACTCT172000.027.7786949
TGAACTC176500.027.2049988
CTATCGG108550.026.17038558-59
GCCCTCT198200.025.753191
CCTTACG111100.025.71416340-41
CGTGCCG110550.025.52522368-69
ACGGTAC111900.025.48792844-45
CTCTTGA188200.025.4632474
GACTATC113450.025.10089556-57
TTGAACT198900.024.7859087
GTCCGTT10900.024.4853741
CCCTCTT213800.024.0804082
CTTGAAC205750.023.9145476
CCGGTAT117700.023.77115672-73
CGATTGA20200.023.7471924
CCTCTTG213350.023.6637123
ATCGGTC120400.023.634160-61
ACTATCG104700.023.61635258-59
Produced by FastQC (version 0.11.5)