FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13951969
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGG301710.21624904699831257No Hit
GGCAAGACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGC284340.20379919135428123No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA272050.19499039884621303No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT259940.1863106203862695No Hit
CGTGCCTCCTGGTCTCAGTATGGCGCTGTCCTGGGTTCTTACAGTCCTGA200900.14399401260137548No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT195480.1401092562633991No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC191780.13745730082972518No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG179470.12863417342742087No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG178830.12817545681186648No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT172010.12328725787736484No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG169360.12138788439108487No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT160450.11500168900891337No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT141850.10167023736936341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT16150.037.9210661
ACTACCG59900.030.1136957
GTAGGTC34300.029.4818742
GTTGACT106150.029.2049561
CTACCGT64050.028.0875328
TTGACTA101000.027.9212382
ACCGTTG23100.026.718683
GACTATT106700.026.5195064
TATAGCC38050.026.476937
GACCGTT24250.025.4507872
CGTGCCT94150.023.9517171
AGTAGGT43300.023.682591
TACTATA42800.023.6275924
ATCACAT81800.023.5052621
TCACATG84800.022.3381232
CATAAAT79300.022.3305281
ACTATAG51350.022.0973955
TGACTAT132100.021.8516563
GCAAGAC135250.021.517552
GCTCGGA52600.021.1594168
Produced by FastQC (version 0.11.5)