FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8613282
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT143060.1660923211384464No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT107940.12531808432604435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT6350.034.4112131
CGGCCCG20350.031.9828668
ACCCGGC21950.030.2969745
TCACCCG27250.024.9278813
CGCCGTA8400.024.8823746-47
CACCCGG26700.024.729734
TAGGTCG7300.022.1244183
CGACCTA7600.021.876124
ACCGTTG10650.021.4095463
CCGTATG22750.021.2977924
GCCGTAT10300.020.98420546-47
ACACGCC74950.020.5342645
GCATTCG16000.020.4854243
CACACGC76500.020.3670564
CTACCGT25650.020.1862181
CATTCGG17100.020.0010244
TAACCCC30650.019.9976259
CGCAAAA30950.019.7990741
CGTATGG24800.019.3452995
CCGGCCC35950.019.2918937
Produced by FastQC (version 0.11.5)