FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8779741
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT145850.1661210735031933No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT108960.12410388871380146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT6600.037.4200941
CGGCCCG20600.027.438318
ACCCGGC21650.024.7833255
CGCCGTA7300.024.39791146-47
ACCGTTG10950.022.981773
CACGCCG10650.021.8466824
TCACCCG25150.021.5222973
CACCCGG25850.021.4913834
CCGTATG20200.020.9207734
GCCGTAT9250.020.53820446-47
GACCGTT11700.020.2908822
GTAGGTC19050.019.6901452
GTAATCG7250.019.6473963
TAACCCC28350.019.4381249
CTACCGT23000.018.9978921
TACACGC12100.018.8353042
CGTATGG22950.018.827725
CGCAAAA31050.018.8143391
ACACGCC78800.018.619645
ACCGTAT23700.018.4315243
Produced by FastQC (version 0.11.5)