FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13205655
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA609860.4618173047834431No Hit
GTCTTGGTGGCGTGCTTCATGTAACCCTGCATGAAGCTGAGAAGGGAGGC145240.1099831852339017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCT200850.031.1188749
GCCCTCT216900.031.1139871
CCCTCTT225900.029.2239152
GTCCGTT17050.028.5120051
CCTCTTG237400.027.8082663
TGAACTC224250.027.808158
TTCCGAT44800.027.6695657
CTCTTGA239400.026.6832034
TTGAACT241650.026.4347557
CTATCGG138200.026.3133258-59
CTTGAAC240000.026.2800796
TCTTGAA245900.026.0744445
ACGGTAC142600.025.61242944-45
CGTGCCG143400.025.3765768-69
CCTTACG145250.025.16154140-41
ATCGCGT7000.025.10859794-95
TCAATCG17500.024.9683388
GACTATC150400.024.19305256-57
TTACGGT151800.024.0758542-43
GTTCCGA50100.023.9840566
Produced by FastQC (version 0.11.5)