FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13731853
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT158470.11540321615735327No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT150030.10925692257264916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACC76400.021.6355786
TCAACCC94100.018.17177
GTTAGTT47150.018.1383111
GCATTCG28650.017.0812113
CATTCGG32550.016.6402534
ACACGCC104100.015.7455785
GTACGTT26150.015.2620781
TCTGTCA125050.014.7799163
CGTGCCT84600.014.7703721
ACGCCTT110250.014.7343227
TACCCTA54450.014.6553319
TACGTTA25800.014.5483412
TTGACTA52150.014.3038012
CGCCTTT114850.014.0616188
CTTGACG21100.013.73578551
CCACACG121600.013.5191253
GACTATT58250.013.4584214
TCAGACG37700.013.3588972
CTTAGCG14150.013.0916858
CACACGC126900.013.0293794
Produced by FastQC (version 0.11.5)