FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13697418
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT157320.11485376295006841No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT148160.10816637120952285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACC71900.019.6198086
GCATTCG31000.019.1568893
GTAATCG8750.017.9177113
CATTCGG33650.017.7893054
CGTGCCT85700.017.5176161
ACACGCC96800.015.99943355
CTACCGT31500.015.8326028
GTTAGTT45600.015.6277041
TCGCATA7800.015.2236567
GTACGTT25650.015.0025951
TACGTTA24450.014.9618512
TCAACCC95350.014.8446217
ACGCCTT106050.014.6457177
GTAGGTC23150.014.5707142
ACTACCG36450.014.0734237
CGCCTTT109400.014.0669928
CCACACG115650.013.4742593
CTAGTAC62350.013.1803794-95
ACGTTAT29900.013.0292483
GTGCCTC115300.013.0206092
Produced by FastQC (version 0.11.5)