FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4726752
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG156960.33206734772630336No Hit
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA85810.181541151302205No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA81770.1729940559606258No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT78140.1653143638591574No Hit
GGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAA77540.16404499326387337No Hit
CCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA51190.10829846795431619No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT50790.10745222089079351No Hit
CCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGT49380.10446919999187604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG12600.032.0405353
CCCGTCG6400.031.2303051
CCGTATC13600.031.0831812
CCCGTAT8500.027.9935511
CGTCGGC6850.025.654443
GCCGTAT9750.025.380821
GTATCGA7900.024.0482944
CGTTCCG9500.023.491758
GTATCGT9600.023.2529454
CGTACGC1252.2627195E-422.7919947
ATCGCGT7250.021.99011694-95
TATCGTT10700.021.7502235
TTCGCGC2005.411657E-721.3674957
CCGGGGC18150.020.9758831
CCCAAAT98800.020.3264921
CCGTCGG9050.019.4190272
TCGACGA55750.019.29633144-45
TAAGCCG56450.019.05866266-67
CGTACGA2500.018.99855454-55
GTCCGTT4800.018.8373281
Produced by FastQC (version 0.11.5)