FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4822886
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG162100.336105808845575No Hit
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA87660.18175839113758857No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA85840.17798471703457225No Hit
GGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAA80080.16604166053271838No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT77220.16011160122797843No Hit
CCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGT51370.1065129882812905No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT51130.10601536092704658No Hit
CCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA50640.10499937174546527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG11950.036.5265433
CCGTATC13700.034.63162
CCCGTAT8100.030.5161
GCCGTAT10500.027.6153031
GTATCGT9300.027.0384354
CGTTCCG9350.026.8843638
GTATCGA7650.024.807744
CGTACGC1151.2958463E-424.745087
CCGGGGC17100.024.462221
CCCGTCG6150.023.9604991
CGTACGA2050.023.1596654-55
ATCGCGT8400.022.94311594-95
TATCGTT11350.021.7368265
CCTATCG2309.181167E-820.6209018
CCCAAAT101300.020.3183171
GCCCTCT53250.019.9064851
TCGACGA55350.019.524944-45
TAAGCCG55950.019.38434466-67
CGTGCCG29950.018.97902168-69
GACGAAA57200.018.94812246-47
Produced by FastQC (version 0.11.5)