FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13217692
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA493640.37346913515612257No Hit
GGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTT201540.15247745219059425No Hit
CGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTT170540.12902403838733723No Hit
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT168930.12780597399303903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCT170650.030.447959
GCCCTCT193000.030.0361711
CCCTCTT203700.028.1658552
TGAACTC190350.027.6211558
CTATCGG113000.026.98878758-59
CCTTACG116550.026.26425740-41
ACGGTAC116200.026.26166744-45
TTGAACT209200.025.8815487
CTCTTGA207100.025.7541224
TCAATCG19150.025.5455658
CTTGAAC207350.025.44826
CGTGCCG120500.025.21114568-69
GACTATC121300.025.14021156-57
CCTCTTG223200.024.8965263
ATCGGTC123300.024.67669160-61
ACTATCG110600.024.3316558-59
GTCCGGT24700.024.3187871
TTACGGT126850.024.1316942-43
TCTTGAA226200.023.852445
TATCGGT113250.023.78349760-61
Produced by FastQC (version 0.11.5)