FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12931719
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA441960.34176430836457244No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG195020.15080748352171897No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA181650.14046856415608783No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT161380.12479392724200083No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC151330.11702233863881514No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA134400.10393049833514013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT48100.038.8777731
GTATCGA48150.038.0687644
CCCGTCG24400.036.3649941
TATCGAA56700.032.3282365
CGTATCG88300.027.0511073
CCGTATC88450.026.092532
ACGACTT21550.024.1290171
TGTCGAG12650.024.0252135
GTTCGAC17100.023.60481528-29
TCGACCG18100.022.43186230-31
ATCGAAG99100.022.3300696
CGATCCG19050.020.94626282-83
CGACCGT17800.020.54227332-33
TCAATCG29900.020.1702068
GTCGGTA7500.019.7179071
CGAAGGC93500.019.7059758
ATCGCGT20700.019.50196694-95
TCGAAGG115200.019.3741727
AAGTTCG21850.018.58226-27
GAACTCT302950.018.3553969
Produced by FastQC (version 0.11.5)