FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12893030
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA435510.3377871609699194No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG192940.14964674711840428No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA179880.13951724303751717No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT159500.12371025274896591No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC147940.11474416797292801No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA136190.10563071675160919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT50550.042.3695261
GTATCGA51450.040.703524
CCCGTCG24250.036.193831
TATCGAA59200.035.5353665
CGTATCG93550.028.7310453
CCGTATC94100.028.3612562
ACGACTT19000.025.8590181
ATCGAAG102550.024.4035916
TGTCGAG13250.023.2957275
CGAAGGC96800.022.0757758
TCGACCG16750.021.97278230-31
GTTCGAC16250.021.9174528-29
TCGAAGG117600.021.2805147
CGATCCG18200.019.8372382-83
CGACCGT17000.019.27610832-33
ACGTTCG11450.019.0779276
GAAGGCC116250.018.790739
GAACTCT302800.018.6154549
CGCGTAT1559.36232E-418.4649451
ATCGCGT19850.018.18222694-95
Produced by FastQC (version 0.11.5)