FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13476532
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT408390.3030379032231734No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG189880.14089678264408081No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT189590.14068159375127073No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT182090.1351163637648024No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC179290.13303867790318755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT21250.047.6026761
TCACCCG94800.044.3853153
ACCCGGC95450.043.8357475
ACCGTTG26000.038.906323
CACCCGG110500.038.2511834
GACCGTT26750.036.9276662
CGGCCCG120550.035.1460088
TAACCCC57750.033.9693879
CGCAAAA64350.030.848811
TAGCATA60350.030.3021289
CTCGATT81450.030.20401648-49
ATTCCGT83350.029.05885152-53
CCAGTAG61700.028.7882235
GTAGCAT63900.028.3206028
CGTTGAC37850.027.4793645
CGGACAC91550.027.4481812-13
CGGACAG91900.027.29570218-19
CGTGGGT89300.027.2824856-57
CCGTTGA38600.027.0677744
CGACCTA16200.026.970564
Produced by FastQC (version 0.11.5)