FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13389841
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT403790.30156444725519893No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT188710.14093520602671833No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG186840.13953862484252053No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT182580.13635710834803788No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC179290.13390002166567921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT20700.050.4778481
TCACCCG97800.043.4180073
ACCCGGC99100.042.657855
ACCGTTG27900.038.4747163
CGGCCCG115150.037.042468
GACCGTT28600.036.7012832
CACCCGG116800.036.5588844
TAACCCC57500.035.0258529
TAGCATA58050.031.6664569
CGCAAAA66950.031.0012631
CTCGATT78750.030.87760248-49
GTAGCAT63800.029.333448
ATTCCGT82200.029.32165152-53
CCAGTAG62900.029.1487055
CGACCTA15000.028.8155064
CGTTGAC38750.027.4573675
CGGACAC91000.027.35412212-13
CGTGGGT89900.026.88947156-57
CGGACAG92150.026.88638518-19
CGTTCTT90300.026.82716276-77
Produced by FastQC (version 0.11.5)