FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13471800
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT405710.30115500527026823No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG189310.1405231669116228No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT187350.13906827595421548No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT179520.13325613503763417No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC178980.13285529773304236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT22650.046.5514681
TCACCCG98200.044.6418383
ACCCGGC98650.044.4393545
ACCGTTG27950.038.404183
CACCCGG117600.037.277594
GACCGTT28450.036.8943862
CGGCCCG121550.036.34168
TAACCCC56000.033.4182939
TAGCATA61100.031.328529
CCAGTAG60700.030.5950535
CGCAAAA63200.030.5862331
CTCGATT86650.030.25417348-49
ATTCCGT87450.029.89594752-53
CGGACAC92350.028.49624412-13
GTAGCAT67000.028.4279568
CCGTTGA37450.028.2817654
CGGACAG95050.027.71485118-19
CGTTGAC38850.027.6299785
CGTTCTT95800.027.02501176-77
ATAGCTC100100.026.42656738-39
Produced by FastQC (version 0.11.5)