FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14104974
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT559590.39673238674527156No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT256490.18184365316802426No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC211910.15023778136705532No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG209410.14846535697265378No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA178670.12667162661909195No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT174620.12380029910016141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT25150.053.8222541
ACCCGGC120950.048.461135
TCACCCG123750.047.9396973
CACCCGG123600.047.6909564
ACCGTTG30000.044.965063
GACCGTT31650.042.7691652
CGGCCCG143300.041.1691558
CGCAAAA70850.039.0155831
TAACCCC75100.036.621949
CCAGTAG62600.036.3450625
TAGCATA68350.034.192389
GTAGCAT70950.032.7380688
CTCGATT109650.031.98588448-49
CTCACCC187050.031.7149722
CGGACAC112900.031.13710412-13
TTAACCC87300.031.1227788
ATTCCGT112450.030.97821652-53
CGGACAG115350.030.80812518-19
CAGTAGC78150.029.5998046
CCGGCCC207600.029.309867
Produced by FastQC (version 0.11.5)