FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13960349
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA249110.1784410977118122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA37850.033.4992374
CCCGTAT41950.032.969361
TATCGAA44500.028.7066065
CCCGTCG23450.027.8626381
CGTATCG70300.023.9807423
CCGTATC71900.023.2489512
TTCGCGC12950.022.7358657
TCAATCG30050.021.6503878
TCGACCG16300.020.39388730-31
GTTCGAC16450.020.35226628-29
ATCGAAG86000.020.0446176
CGATCCG17350.018.89154682-83
TCGAAGG102850.017.2224167
TCTACGG54600.017.0471977
CGTACGC65050.016.7177475
GTCCGTT9800.016.5461651
CCGTACG66300.016.4025554
CTTCGCG18550.016.3842146
GGTCTAC58800.015.9103045
ACGACTT22800.015.8972961
Produced by FastQC (version 0.11.5)